Abstract The anisotropy of shear resistance depending on friction direction can be selectively utilized in geotechnical structures. For instance, deep foundations and soil nailing, which are subject to axial loads, benefit from increased load transfer due to greater shear resistance. In contrast, minimal shear resistance is desirable in applications such as pile driving and soil sampling. Previous studies explored the shear resistance by interface between soil and surface asperities of a plate inspired by the geometry of snake scales. In this study, the interface friction anisotropy based on the load direction of cones with surface asperities is evaluated. First, a laboratory model chamber and a small-scale cone system are developed to quantitatively assess shear resistance under two load directions (penetration ⟶ pull-out). A preliminary test is conducted to analyze the boundary effects for the size of the model chamber and the distance between cones by confirming similar penetration resistance values at four cone penetration points. The interface shear behavior between the cone surface and the surrounding sand is quantitatively analyzed using cones with various asperity geometries under constant vertical stress. The results show that penetration resistance and pull-out resistance are increased with a higher height, shorter length of asperity and shearing direction with a decreasing height of surface asperity. Keywords: interface friction anisotropy; model chamber; small-scale cone system; bio-inspired surface; boundary effect; penetration and pull-out resistance