Integrating advanced energy harvesting technologies into conventional textiles has been envisioned as a sustainable and reliable power source in wearable electronics. However, conventional power textiles often rely on complex multilayer structures, compromising wearing comfort, mechanical stability, and output performance. Here, a monolayer power textile that efficiently harvests human mechanical energy in situ by embedding a single conductive fiber into an insulating textile, while maintaining wearing comfort and showing excellent washability and mechanical stability is introduced. This innovative design leverages the interfacial electrostatic breakdown effect between textiles, overcoming conventional limitations of electrostatic breakdown on output performance. This results in a high-power density and a safe high electrostatic voltage (6 kV), which uniquely offers air purification and antibacterial benefits. Furthermore, a quantified triboelectric series including 64 textile pairs is established for guiding applications. This work provides a novel strategy for designing multifunctional power textiles and addresses key challenges in wearable electronics.