Abstract The use of silver-based antimicrobial materials has been growing recently. Considering the threat of developing silver-resistant bacteria, it is essential to address the endurance of such materials and the amount of silver released into the environment. Here we report on a durable, antibacterial Ag/TiB x nanocomposite coating prepared by conventional magnetron sputtering. The coating consists of fine Ag clusters embedded in extremely hard, wear-resistant overstoichiometric TiB x, which serves as a protective matrix. The highest E. coli growth inhibition of 97% is observed for the coating containing 24 at.% of Ag. A strong antibacterial effect is also maintained after 45 days of immersion in the Luria–Bertani + 5% HNO 3 solution. Despite a marked hardness decrease from 40 GPa for TiB x to 6.4 GPa for Ag/TiB x with 28 at.% of Ag, the coating maintains a good specific wear rate of 6 × 10 −5 mm 3/Nm. Moreover, the addition of Ag, which acts as a solid lubricant, decreases the coefficient of friction from 0.77 to 0.35, even at room temperature. Thanks to the combination of antibacterial properties and enhanced wear resistance, such material can find application as a protective coating for cutlery, door handles, water taps, and other daily-used objects in public areas. Keywords: antibacterial; hard coating; silver; titanium diboride antibacterial; hard coating; silver; titanium diboride The use of silver-based antimicrobial materials has been growing recently. Considering the threat of developing silver-resistant bacteria, it is essential to address the endurance of such materials and the amount of silver released into the environment. Here we report on a durable, antibacterial Ag/TiB x nanocomposite coating prepared by conventional magnetron sputtering. The coating consists of fine Ag clusters embedded in extremely hard, wear-resistant overstoichiometric TiB x, which serves as a protective matrix. The highest E. coli growth inhibition of 97% is observed for the coating containing 24 at.% of Ag. A strong antibacterial effect is also maintained after 45 days of immersion in the Luria–Bertani + 5% HNO 3 solution. Despite a marked hardness decrease from 40 GPa for TiB x to 6.4 GPa for Ag/TiB x with 28 at.% of Ag, the coating maintains a good specific wear rate of 6 × 10 −5 mm 3/Nm. Moreover, the addition of Ag, which acts as a solid lubricant, decreases the coefficient of friction from 0.77 to 0.35, even at room temperature. Thanks to the combination of antibacterial properties and enhanced wear resistance, such material can find application as a protective coating for cutlery, door handles, water taps, and other daily-used objects in public areas.